GEN

Streamlining Multiplex PCR Analysis

Multiplex PCR is a difficult technique that allows amplification of two or more products in parallel in a single reaction tube. This technology was first described by Chamberlain et al., in 1988, and ...
The Scientist

Digital PCR Explained: Absolute Quantification Advantages and Applications

Digital PCR, or dPCR, is a molecular biology technique that detects and quantifies nucleic acid targets. By partitioning a sample into many individual reactions, scientists can measure whether a ...
GEN

PCR @ 30: 30 Years Young and Still Evolving

It was 30 years ago when Kary Mullis conceived the chain reaction that looked possible by adding polymerase to a DNA molecule in the presence of short oligonucleotide primers and nucleotide ...
Nature

A Single-Tube PCR Test for the Diagnosis of Angelman and Prader-Willi Syndrome Based on Allelic Methylation Differences at the SNRPN Locus

The Angelman syndrome (AS) and the Prader-Willi syndrome (PWS) are distinct neurogenetic disorders which are caused by a deficiency of maternal (AS) or paternal (PWS) contributions for chromosome 15.
The Scientist

A Novel Polymerase Reduces Stutter in Forensic DNA Analysis

When TV shows like CSI: Crime Scene Investigation portray DNA processing in the forensic lab, the results appear instantly with a DNA match wrapping up the case in hours. However, the ...